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Hua Li
Hua Li
Dalian University of Technology
Key Laboratory Of Mariculture And Biotechnology,
China
Publications
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Research Article
Modified a Colony Forming Unit Microbial Adherence to Hydrocarbons Assay and Evaluated Cell Surface Hydrophobicity and Biofilm Production of Vibrio scophthalmi
Author(s): Guo Qiao, Hua Li, De-Hai Xu and Soo Il Park*Guo Qiao, Hua Li, De-Hai Xu and Soo Il Park*
Vibrio scophthalmi has been considered as an opportunistic pathogen of the flat fish. There is little information available on V. scophthalmi adhesion to the host, an important step in the initial infection process. The objectives of this study were to (1) develop a modified Microbial Adherence to Hydrocarbons (MATH) assay using Colony Forming Unit (CFU) method and evaluate the cell surface hydrophobicity of V. scophthalmi , (2) detect slime layer and biofilm production of V. scophthalmi and (3) investigate the motility and antibiotic susceptibility associated with biofilm production of V. scophthalmi . The results showed that cell surface hydrophobicity of V. scophthalmi was moderate by Salt Aggregation Test (SAT) and MATH assay. The fraction partitioned to the hydrocarbon phase (%Adh) was determined significantly higher by modified CFU MATH assay than that by class.. View More»
DOI: 10.4172/2155-9597.1000130