Clinical Microbiology: Open Access

Hassan Dana

Publications

• Research Article
  Cloning and Expression of C2 and V Domains of ALCAM Protein in E. coli BL21 (DE3)
  Author(s): Hassan Dana, Ali Mazraeh, Ghanbar Mahmoodi Chalbatani, Vahid Marmari, Habibollah Mahmoodzadeh, Ali Ghamari, Fateme Moazzen, Mohammad Ebrahimi and Narges MehmandoostHassan Dana, Ali Mazraeh, Ghanbar Mahmoodi Chalbatani, Vahid Marmari, Habibollah Mahmoodzadeh, Ali Ghamari, Fateme Moazzen, Mohammad Ebrahimi and Narges Mehmandoost
  
  Introduction: ALCAM as a glycoprotein is a member of the immunoglobulin family and plays an important role in cell growth, survival and motility. This protein also contributes to the development of tumour invasion in colorectal and breast cancers and is typically considered as a cancer stem cell marker. ALCAM as a cell-surface antigen, with high expression in some cancers as colorectal and breast, has the potential to diagnose and treat these cancers. Objectives: As with other membrane proteins, ALCAM represents a potential target for therapy of colorectal and breast cancers, therefore, this study is aimed to clone and express C2 and V domains to be used in practical, diagnostic and remedial plans. Methods: In this study, the sequence of C2 and V domains were optimized for expression in prokaryotic host using online tools and cloned in pET-28a expression plasmids. E. coli BL21 (DE3) c.. View More»
  DOI: 10.4172/2327-5073.1000271

  Abstract PDF