Gene Technology
Open Access
ISSN: 2329-6682
ISSN: 2329-6682
Hasan Hafezian
Italy
Research Article
Genetic Analysis of SNPs in GH, GHR, IGF-I and IGFBPII Genes and their Association with Some Productive and Reproductive Traits in Native Breeder Hens
Author(s): Hamed Kazemi, Mohammad Rezaei, Hasan Hafezian, Ghodrat Rahimi Mianji and Mojtaba NajafiHamed Kazemi, Mohammad Rezaei, Hasan Hafezian, Ghodrat Rahimi Mianji and Mojtaba Najafi
The aim was to study the polymorphisms in GH, GHR, IGF-I and IGFBPII genes and their association with productive and reproductive traits. DNA was extracted from blood samples of 380 breeder hens of Mazandaran native fowls breeding station by modified salting out method. For genotyping, PCR products of GH, GHR, IGF-I and IGFBPII loci were digested with specific restriction enzymes. We found six genotypes of AA, BB, CC, AB, AC and BC with the frequency of 0.10, 0.01, 0.36, 0.07, 0.34 and 0.12 in GH, two haploid alleles and genotypes of B+ and B- with the frequency of 0.72 and 0.28 in GHR, three genotypes of BB, Bb, bb with the frequency of 0.49, 0.44 and 0.07 in IGF-I and three genotypes of CC, CT and TT with the frequency of 0.14, 0.62 and 0.24 in IGFBPII loci, respectively. Significant associations were found between SNPs in IGF-I locus and egg number at 120-270 and 345-375 days of ag.. Read More»
DOI:
10.4172/2329-6682.1000145