Journal of Blood Disorders & Transfusion

Hans-Joachim Freisleben

Publications

• Research Article
  Comparison of Effective α-Tocopherol Concentrations Ameliorating Reduced Glucose Transport and Counteracting t-Butyl hydroxyperoxide- Induced Lipid Peroxidation in Isolated Erythrocytes from β-Thalassemic Patients
  Author(s): Chris Adhyanto, Marice Sihombing, Hans-Joachim Freisleben, Rondang R. Soegianto, Septelia I. Wanandi and Ridwan RahmawatiChris Adhyanto, Marice Sihombing, Hans-Joachim Freisleben, Rondang R. Soegianto, Septelia I. Wanandi and Ridwan Rahmawati
  
  In thalassemia patients Red Blood Cell (RBC) membrane is the major target of oxidative stress caused by hemichrome radicals. Moreover, RBC membranes from thalassemic patients are more susceptible to oxidative stress, which causes lipid peroxidation, membrane rigidity, and dysfunction of membrane proteins. We investigated hemoglobin-free isolated RBC membranes (ghosts) from 28 β-thalassemia major patients and from 24 normal blood samples. Our research aimed to determine the effective concentrations of racemic α-tocopherol i) counteracting experimental oxidative stress induced by 2mM tertiary butylhydroperoxide (t-BHP) and ii) ameliorating impaired glucose transport in RBC ghosts from thalassemia patients. Reduced glucose transport in thalassemic ghosts was ameliorated significantly by tocopherol with a maximum effect at 75 ppm concentration. Furthermore, ghosts were pre-incub.. View More»
  DOI: 10.4172/2155-9864.1000189

  Abstract PDF