Clinical Microbiology: Open Access

Habib Abdul Hakim Esa

Publications

• Research Article
  Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit for Detection of IgM Antibody against Leptospira in Human Sera for Patients admitted in Malaysian Hospitals
  Author(s): Habib Abdul Hakim Esa, Fairuz Amran and Nurul Atiqah Noor HalimHabib Abdul Hakim Esa, Fairuz Amran and Nurul Atiqah Noor Halim
  
  Leptospirosis is an infectious zoonotic disease caused by corkscrew shaped of pathogenic Leptospira spp. Early diagnosis is important as the disease can be treated with antibiotic to prevent complications. The gold standard of leptospirosis diagnosis is microscopic agglutination test (MAT). However, the test is laborious and time consuming. This study aimed to evaluate whether a commercial ELISA (enzyme-linked immunosorbent assay) kit by Virion- Serion can demonstrate antibody against leptospires for accurate and rapid screening for leptospirosis. A total of 212 serum samples were tested with Virion-Serion Classic ELISA IgM, which was earlier collected from patient with clinical manifestation suggestive of leptospirosis in Malaysia and confirmed with MAT. The results were analyzed into two categories. In the first category all samples that were positive with MAT but detected as interm.. View More»
  DOI: 10.4172/2327-5073.1000307

  Abstract PDF