Research Article
Histological and Transcriptional Expression differences between Diabetic Foot and Pressure Ulcers
Author(s): Yssel Mendoza-Mari, Calixto Valdés-Pérez, Elena Rodríguez-Corrales, José Suárez-Alba, Ariana García-Ojalvo, Diana García del Barco, Gerardo Guillén-Nieto, Luis Herrera-Martínez and Jorge Berlanga-AcostaYssel Mendoza-Mari, Calixto Valdés-Pérez, Elena Rodríguez-Corrales, José Suárez-Alba, Ariana García-Ojalvo, Diana García del Barco, Gerardo Guillén-Nieto, Luis Herrera-Martínez and Jorge Berlanga-Acosta
Decubitus and diabetic foot ulcers remain as important clinical challenges with significant socioeconomic impact. Both are individual forms of chronic wounds with diverse proximal ethiopathogenic triggers. This study aimed to characterize and compare the main histological features as the transcriptional expression profile of a set of wound-healing relevant genes of the ulcers’ granulation tissue. Following patients’ consent, biopsies were collected from sacrolumbar pressure ulcers (N=5, stage IV) and diabetic foot ulcers (N=9, both of neuropathic and ischemic origin) with clean, non-infected granulation tissue. Biopsies fragments were processed for histological analysis and for RNA extraction and subsequent transcriptional expression characterization via RT-PCR. The group of targeted genes included cell proliferation control, extracellular.. View More»
