Cardiovascular Pharmacology: Open Access
Open Access
ISSN: 2329-6607
ISSN: 2329-6607
Fumitaka Nakamura
Japan
Research Article
Thermo-Sensitive Transient Receptor Potential Vanilloid (TRPV) Channels Regulate IL-6 Expression in Mouse Adipocytes
Author(s): Hironobu Kikuchi, Gaku Oguri, Yumiko Yamamoto, Nami Takano, Tomofumi Tanaka, Masao Takahashi, Fumitaka Nakamura, Tatsuya Yamasoba, Issei Komuro, Syotaro Obi and Toshiaki NakajimaHironobu Kikuchi, Gaku Oguri, Yumiko Yamamoto, Nami Takano, Tomofumi Tanaka, Masao Takahashi, Fumitaka Nakamura, Tatsuya Yamasoba, Issei Komuro, Syotaro Obi and Toshiaki Nakajima
Background: Hyperthermia may affect adipose cell functions and then adipogenesis. Thermo-sensitive transient receptor potential (TRPs) proteins such as TRPV1-4 play vital roles in cellular functions. We investigated the expression of thermo-sensitive TRPVs and their roles on IL-6 production in mouse 3T3-L1 preadipocytes and differentiated adipocytes.
Methods: The conventional and quantitative real-time RT-PCR analysis, western blots, fluorescence activating cell sorting, immunocytochemical staining and [Ca2+]i measurements using fura-2 AM were performed.
Results: The conventional and real-time RT-PCR analysis showed the existence of TRPV1, 2 and 4 expressions. Western blotting and immunocytochemical staining also showed the existence of TRPV1, TRPV2 and TRPV4 transcript in 3T3-L1 preadipocytes and differentiated adipoc.. Read More»
DOI:
10.4172/2329-6607.1000156