Farrokhi N
Department of Agronomy and Plant Breeding/ Biotechnology Research Group, Faculty of Agriculture,
Shahrood
West Indies
Research Article
Computational Identification, Characterization and Analysis of Conserved miRNAs and their Targets in Amborella Trichopoda
Author(s): Hajieghrari B, Farrokhi N, Goliaei B and Kavousi KHajieghrari B, Farrokhi N, Goliaei B and Kavousi K
MicroRNAs (miRNAs) are single stranded non-coding endogenous small RNAs of about 22 nucleotides, which are directly involved in regulating gene expression at post transcriptional level. miRNAs play key roles in development and response to biotic and abiotic stresses. Homology searches allow identification of new miRNAs due to their relative high conservation in plant species. Here, miRNAs were identified for Amborella trichopoda. Known and unique plant miRNAs from miRBase were BLAST-searched against Expressed Sequence Tag (EST) and Genomic Survey Sequence (GSS) in A. trichopoda. All candidate sequences with appropriate fold back structure were screened by a series of miRNA filtering criteria. Finally, we identified and analysed conservation of 5 potential conserved miRNAs belonging to 5 miRNA gene families from ESTs as well 82 newly identified miRNAs dependant 39 miRNA families from G.. View More»
DOI:
10.4172/2153-0602.1000168