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Ezebuiro Victor
Ezebuiro Victor
Nigeria
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Research Article
Role of Plasmid-Borne Genes in the Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Consortium of Aerobic Heterotrophic Bacteria
Author(s): Isiodu GG, Stanley OH, Ezebuiro Victor and Okerentugba POIsiodu GG, Stanley OH, Ezebuiro Victor and Okerentugba PO
Background Aim: Certain plasmids play an important role in adaptation of natural microbial populations to oil and other hydrocarbons. This research investigated the role of plasmid-borne genes in the biodegradation of polycyclic aromatic hydrocarbons (PAHs) by a consortium of aerobic heterotrophic bacteria (AHB). Methods: Physicochemical and microbiological characteristics of the water sample were analyzed using standard methods. AHB with the capacity for biodegradation were isolated from hydrocarbon polluted water from Bodo Creek using Bushnell Haas Agar. The positive isolates were subjected to plasmid profiling and curing. Three treatments: cured, uncured and control were used in the study. Curing was achieved with acridine orange. The degradation experiment was carried out in a 10 L glass bioreactor and monitored for .. View More»
DOI: 10.4172/2157-7463.1000264