Eugênio V. Santos Neto
Brazil
Research Article
Taxonomic Diversity and Biodegradation Potential of Bacteria Isolated from Oil Reservoirs of an Offshore Southern Brazilian Basin
Author(s): PatrÃcia F. Lopes- Oliveira, Suzan P. Vasconcellos, Célio F. F. Angolini, Georgiana F. da Cruz, Anita J. Marsaioli, Eugênio V. Santos Neto and Valéria M. OliveiraPatrícia F. Lopes- Oliveira, Suzan P. Vasconcellos, Célio F. F. Angolini, Georgiana F. da Cruz, Anita J. Marsaioli, Eugênio V. Santos Neto and Valéria M. Oliveira
This study aimed at the taxonomic characterization of a collection with 98 bacteria isolated from oil and formation water samples from petroleum reservoirs of the Campos Basin (Brazil), as well as the evaluation of their degradation potential of petroleum biomarkers. The genomic DNA extracted from all isolates was employed in PCR reactions for amplification of the 16S rRNA gene and subsequent screening by ARDRA (Amplified Ribosomal DNA Restriction Analysis), in order to detect potentially distinct taxonomic groups. Further 16S rRNA gene sequencing and phylogenetic analysis of 39 isolates representing different ribotypes revealed that these isolates belonged to 10 different genera, encompassing Marinobacter, Halomonas, Citreicella, Stenotrophomonas, Achromobacter, Bacillus, Staphylococcus, Micrococcus, Kocuria and Streptomyces, affiliated to the three phyla Proteobacteria, Firmicutes a.. View More»
DOI:
10.4172/2157-7463.1000132