Journal of Allergy & Therapy

Cui Zhou

Publications

• Research Article
  Allergenicity Assessment of a Genetically Modified Protein-Recombinant Human Lactoferrin
  Author(s): Cui Zhou, Na Sun, Jing Wang, Jing Lu, Jing Tian, Richard E Goodman, Ning Li, Huilian Che and Kunlun HuangCui Zhou, Na Sun, Jing Wang, Jing Lu, Jing Tian, Richard E Goodman, Ning Li, Huilian Che and Kunlun Huang
  
  Background: Recombinant human lactoferrin (rhLF) has previously suggested serving as food additive due to the natural iron binding properties that provide anti-microbial activity. Recombinant cows have been produced that express hLf in milk. However, the potential allergenicity of hLf has not been previously assessed. This research is conducted to evaluate the potential allergenicity of rhLF as a prerequisite for food use. Methods: A comparison was made of the bioactivity, physicochemical properties and glycosylation profile between rhLF and natural hLF. The amino acid sequence of hLf was compared to known allergens. Additionally, the stability of hLf in pepsin and a human serum IgE test was conducted. Results: The amino acid identity between rhLF and the minor allergen bovine lactoferrin was 71.4%. However, every human is exposed to hLf constantly without demonstrated.. View More»
  DOI: 10.4172/2155-6121.S3-002

  Abstract PDF