Journal of Data Mining in Genomics & Proteomics

Brian C. Tooker

Publications

• Research Article
  Attempted Validation of Surface Enhanced Laser Desorption Ionization-Time of Flight Derived Kinesin Biomarkers in Malignant Mesothelioma
  Author(s): Stephen M. Brindley, Brian C. Tooker, Harvey I. Pass and Lee S. NewmanStephen M. Brindley, Brian C. Tooker, Harvey I. Pass and Lee S. Newman
  
  Surface-enhanced Laser Desorption/Ionization Time-of-flight (SELDI-TOF) mass spectrometry allows researchers to search proteomes for biomarkers of disease. Validating SELDI-TOF results using traditional methodologies has yielded mixed results. Real-time PCR, ELISA and Western blot assays were utilized in an attempt to confirm the link between asbestos related cancers and kinesin family proteins, KIF5A and KIF18A. These proteins had been identified using SELDI-TOF and Classification and Regression Tree Analysis (CART) in serum of patients with asbestos related cancers. When well-characterized asbestos fibers were used to challenge mesothelial cell lines, mixed results in KIF5A and KIF18A RNA expression were observed. Western blot analysis comparing protein levels in mesothelioma cell lines versus transformed mesothelial cells yielded inconclusive results, as did Western blot analyses o.. View More»
  DOI: 10.4172/2153-0602.S1-002

  Abstract PDF