Journal of Blood Disorders & Transfusion

Bertholf M

Publications

• Research Article
  Mechanical Fragility as a Potential Time-Independent Measure of Membrane Integrity among Stored RBC Units
  Author(s): Tarasev M, Alfano K, Chakraborty S, Bertholf M and Zubair ATarasev M, Alfano K, Chakraborty S, Bertholf M and Zubair A
  
  Background and Objectives: Previous studies have shown that storage causes RBC membrane damage and subsequent potassium leakage to extracellular environment, with the effects exacerbated by RBC irradiation. While damage to RBC appears to worsen with storage time (ST), ST alone has not been shown to fully account for this phenomenon. It is therefore important to study the extent to which other time-independent factors can affect RBC membrane integrity. RBC mechanical fragility (MF) is evaluated as a surrogate measure of RBC membrane integrity due to its potential to reflect aggregate biochemical and biomechanical changes associated with storage. Materials and methods: Samples from 45 units non-irradiated and 58 units of irradiated leuko reduced RBC units were subjected to shear stress using a bead mill at different durations at a fixed intensity (50 Hz); induced hemolysis was.. View More»
  DOI: 10.4172/2155-9864.1000139

  Abstract PDF