Journal of Clinical & Experimental Pharmacology

Bennett J Giardina

Publications

• Review Article
  The Non-classical Pathway is the Major Pathway to Secrete Proteins in Saccharomyces cerevisiae
  Author(s): Kathryn R Stein, Bennett J Giardina and Hui-Ling ChiangKathryn R Stein, Bennett J Giardina and Hui-Ling Chiang
  
  Protein secretion is a fundamental process in all living cells. Proteins that contain the ER signal are secreted by the classical pathway, whereas proteins without the ER sequence are secreted via the non-classical pathway. Recent evidence indicates that a large number of signal-less proteins including metabolic enzymes, transcriptional factors, translation factors, heat shock proteins, and anti-oxidant proteins are secreted by a variety of cells that range from bacteria to human. Furthermore, the secretion of gluconeogenic enzymes has been observed in bacteria, fungi, parasites, and mammalian cells. Gluconeogenic enzymes are secreted into the periplasm when Saccharomyces cerevisiae are grown in low glucose. The distribution of gluconeogenic enzymes in the periplasm/ extracellular fraction of Saccharomyces cerevisiae was observed by immuno-TEM, confirmed with an extraction protocol, and .. View More»
  DOI: 10.4172/2161-1459.1000155

  Abstract PDF