Journal of Tumour Research & Reports

Bengt Widegren

Publications

• Research Article
  A GFP Positive Glioblastoma Cell Line NS1 A New Tool for Experimental Studies
  Author(s): Henrietta Nittby, Karolina Förnvik, Jonatan Ahlstedt, Crister Ceberg, Peter Ericsson, Bertil R Persson, Gunnar Skagerberg, Bengt Widegren, Zhongtian Xue and Leif G SalfordHenrietta Nittby, Karolina Förnvik, Jonatan Ahlstedt, Crister Ceberg, Peter Ericsson, Bertil R Persson, Gunnar Skagerberg, Bengt Widegren, Zhongtian Xue and Leif G Salford
  
  One of the key problems when treating patients with malignant gliomas is that despite being able to remove the major bulk of the tumour, tumour cells may have already spread to distant sites of the brain. The aim of the present study is to develop a GFP positive tumour cell line (named NS1) where these cells could be identified for future treatment studies in fully immunocompetent animals. Five homozygous GFP positive Fischer 344 rats were treated with ENU during pregnancy. Of the offspring, 19 rats developed CNS tumours. Of these, we chose those with tumours growing intracerebrally for further culturing. The tumour cells were transplanted to new recipients. Out of these, we have defined a cell line (NS1), which originated in pons, and reliably generates tumours when transplanted s.c. and i.c. Our new GFP positive cell line was transplanted into GFP negative Fischer 344 rats. The tumo.. View More»
  DOI: 10.4172/2475-3203.1000101

  Abstract PDF