Biology and Medicine

Azam Rahimpour

Publications

• Research Article
  Stable Expression of Anti-CD52 Monoclonal Antibody Using a Bicistronic Vector System
  Author(s): Azam Rahimpour, Omid Mohammadian, Fatemeh Naderi, Hadi Bayat, Meysam Omidi and Morvarid PeyrovanAzam Rahimpour, Omid Mohammadian, Fatemeh Naderi, Hadi Bayat, Meysam Omidi and Morvarid Peyrovan
  
  The efficient development of stable monoclonal antibody-producing mammalian cells is a tedious and time-consuming process due to the structural complexity of these molecules. The ratio of the light-chain to heavy-chain expression is critical for the assembly and successful production of functional antibodies. Different vector-design strategies have been employed for the optimal expression of monoclonal antibodies in mammalian cells. In the current study, a bicistronic expression based on the encephalomyocarditis virus internal ribosomal entry site (ECMV IRES) element was used for the development of Chinese hamster ovary (CHO)–stable cell pools expressing an anti-CD52 antibody. The successful expression of the monoclonal antibody in CHO cells was achieved with the maximum titer of 20 μg/l. Our results here show that IRES-mediated bicistronic expression is an efficient method f.. View More»
  DOI: 10.4172/0974-8369.1000341

  Abstract PDF