Avijit Roy
Citrus Research and Education Center, 700, Experiment Station Road, Lake Alfred, Florida 33850
Tanzania
Review Article
A Case Study on Discovery of Novel Citrus Leprosis Virus Cytoplasmic Type 2 Utilizing Small RNA Libraries by Next Generation Sequencing and Bioinformatic Analyses
Author(s): Avijit Roy, Jonathan Shao, John S Hartung, William Schneider and RH BrlanskyAvijit Roy, Jonathan Shao, John S Hartung, William Schneider and RH Brlansky
The advent of innovative sequencing technology referred to as “Next-Generation” Sequencing (NGS), provides a new approach to identify the ‘unknown known’ and ‘unknown unknown’ viral pathogens without a priori knowledge. The genomes of plant viruses can be rapidly determined even when occurring at extremely low titers in the infected host. The method is based on massively parallel sequencing of the population of small RNA molecules 18-35 nucleotides in length produced by RNA silencing host defense. Improvements in chemistries, bioinformatic tools and advances in engineering has reduced the costs of NGS, increased its accessibility, and enabled its application in the field of plant virology. In this review, we discuss the utilization of the Illumina GA IIX platform combined with the application of molecular biology and bioinformatic tools for the disc.. View More»
DOI:
10.4172/2153-0602.1000129