Ariel G. Garro
Argentina
Research Article
Antibodies can be Spontaneously Loaded onto Monosialoganglioside Micelles Containing Oncological Drugs
Author(s): Ariel G. Garro, Roxana V. Alasino, Victoria Leonhard, Valeria Heredia and Dante M. BeltramoAriel G. Garro, Roxana V. Alasino, Victoria Leonhard, Valeria Heredia and Dante M. Beltramo
Recently, we demonstrated that GM1 micelles transport paclitaxel and doxorubicin with high efficiency. When this GM1-drugs complex is incubated with whole serum, albumin was the only one protein that binds spontaneously to form GM1-drug-albumin complex. Here, we show that, under specific physicochemical conditions, these micelles interact with antibodies forming GM1-IgG complexes. The load of IgG in GM1 reaches a maximum at ratios of 1/4 (w / w) incubating to 4.5 and preheating the micelles of GM1 at 55-60°C. The IgG of the GM1-IgG complex obtained under these experimental conditions retains the biological activity against the soluble and cellular antigens and is not displaced from the micelles in the presence of albumin, the main competitive binding protein. Treatment of GM1-IgG with pepsin, does not show the breakage of the IgG like control of free IgG, suggesting that IgG is de.. View More»
DOI:
10.35248/2157-7439.19.10.532