Journal of Stem Cell Research & Therapy

Ali El Othmani

Publications

• Research Article
  Cryopreservation Impacts Cell Functionality of Long Term Expanded Adipose-Derived Stem Cells
  Author(s): Ali El Othmani, Sabrina Rouam, Anass Abbad, Chaimaa Erraoui, Sara Harriba, Hassan Boukind, Jalal Nourlil, Gabriel Malka and Loubna MaziniAli El Othmani, Sabrina Rouam, Anass Abbad, Chaimaa Erraoui, Sara Harriba, Hassan Boukind, Jalal Nourlil, Gabriel Malka and Loubna Mazini
  
  Objective: Adipose-Derived Stem Cells (ADSCs) are less immunogenic cells and have a heterogenic cytokine secretion profile making them a better candidate for cell-based immunotherapy. Even innately or stimulated, Interleukin-6 (IL-6) and Toll-like Receptor 2 (TLR2) secreted by ADSC were modulated and led to different inflammatory mechanism pathways through different inflammatory factors secretion. These properties can be very useful in the treatment of inflammation-associated chronic diseases. To be used, ex-vivo ADSC expansion is a critical issue prior to transplantation or cryopreservation. Functional cell changes have been reported during culture expansion being susceptible to interact with freezing/thawing effects leading to doubt on cell therapeutic outcomes. The aim of this study is to identify the effect of freezing/thawing at the different time point of expans.. View More»
  DOI: 10.4172/2157-7633.1000445

  Abstract PDF