Journal of Blood Disorders & Transfusion

Aleksey A. Nakorchevsky

Publications

• Research Article
  Detection of Low Level Mixed Chimerism Using High Throughput SNP Genotyping
  Author(s): Aleksey A. Nakorchevsky, Eunice Flores, Li Xiangyang, Tao Hong and Anders O.H. NygrenAleksey A. Nakorchevsky, Eunice Flores, Li Xiangyang, Tao Hong and Anders O.H. Nygren
  
  Recipients of allogeneic bone marrow transplants (BMT) or stem cell transplants (SCT) require clinical monitoring to allow for early diagnosis of such post-transplant adverse effects as rejection, graft vs. host disease (GVHD), or a malignancy relapse. Triaging of the transplant recipients in clinical settings is achieved by monitoring the Minimal Residual Disease (MRD) and measuring the amount of mixed chimerism in peripheral blood lymphocytes (PBL). While MRD monitoring involves detection of the malignancy-specific markers, measuring the extent of mixed chimerism can be achieved via general PCR-based methods. We have developed a SNP genotyping method to detect low levels of mixed chimerism in PBL and genomic DNA. Sensitivity is achieved by measuring a cumulative skew in genotyping data across a cohort of 92 independent SNP markers. This method showed a s.. View More»
  DOI: 10.4172/2155-9864.1000340

  Abstract PDF