Adi J
Tanzania
Research Article
High Protein/Fish Oil Diet Prevents Hepatic Steatosis in NONcNZO10 Mice; Association with Diet/Genetics-regulated Micro-RNAs
Author(s): Adi A, Adi J, Lassance-Soares RM, Kurlansky P, Yu H and Webster KAAdi A, Adi J, Lassance-Soares RM, Kurlansky P, Yu H and Webster KA
Objective: NONcNZO10 (NZ10) mice are predisposed to obesity and develop type 2 diabetes (T2D) and hepatic steatosis even when maintained on a control diet (CD) of 6% fat. Studies were designed to determine whether this extreme susceptibility phenotype could be alleviated by diet and if so the molecular targets of diet. Methods: NZ10 and SWR/J (SWR) control mice were fed a CD or a test diet of high protein and fish oil (HPO) for 19 weeks and then analyzed for steatosis, blood chemistry, hepatic gene and micro-RNA expression. Results: HPO diet prevented steatosis, significantly increased serum adiponectin and reduced serum cholesterol and triglycerides only in NZ10 mice. The HPO diet repressed hepatic expression of fatty acid metabolic regulators including PPARγ, sterol regulatory element-binding protein-c1, peroxisome proliferator-activated receptor gamma co-acti.. View More»
