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Article Name - The Application of CRISPR/Cas9 System in the Basidiomycete Ganoderma lucidum

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  • Keywords Ganoderma lucidum, CRISPR/Cas9, Gene modification, Gene function analysis ... 2023-12-11
  • Introduction G. lucidum can produce various bioactive products (ganoderic acids, polysaccharides, sterols, etc.), which have limitless potential in the food and biological medicine [1]. While low yield and ... 2023-12-11
  • Literature Review In this review, we summarize the establishment of CRISPR/ Cas9 and its biotechnological application in G. lucidum. What’s more, the potential challenges of CRISPR/Cas9 are discussed and it ... 2023-12-11
  • Discussion For the CRISPR-Cas9 system with in vitro transcribed sgRNA, the sgRNA was usually synthesized by a costly kit [9]. Besides, tedious preparing process of sgRNA and Cas9 protein was timeconsuming ... 2023-12-11
  • Conclusion In conclusion, two kinds of CRISPR/Cas9 systems (in vitro transcribed sgRNA and in vivo transcribed sgRNA) were established and utilized for the biotechnological application in G. lucidum. In fu ... 2023-12-11
  • Ethics Declaration No ethical approval was required as this is a review article with no original research data. ... 2023-12-11
  • Conflict Of Interest Statement All authors have no competing interests. ... 2023-12-11
  • Authors' Contribution Ping-An Wang conceived the work and drafted the manuscript. ... 2023-12-11
  • References Wang PA, Zhong JJ. Strain breeding and gene complementation strategies for filamentous fungi. Process Biochem. 2023;128:181-189. [Crossref][Google Scholar] Xiao H, Zhong JJ. Producti ... 2023-12-11