Abstract

Vaccination and Biopharming Technology

Rana Khalid Iqbal, Javiera Khalid, Ayesha Tayyab, Mamoona Sattar, Farina Ansar, Sidra Aslam Nimra Kunwal and Muhammad Imran Qadir

Vaccination is very essential medical aid to prevent the onset of disease with the development of science and technology many advances occur in vaccination technology biopharming technology is most recent technology serving as a platform for vaccine development plant vaccine either in the form of capsules or injections presently and edibles for the future are most preferable for under developed countries and automated biopharming factories are working in this respect, the expression of proteins depends on the selected host system, intrinsic properties of desired protein. Nuclear transformation is most suitable and well-studied of all, biolistic transformation technology removes the need of vectors, but targeting is compromised. Stability of recombinant DNA can be maintained by over-expression of desired protein and optimization is achieved by the selection of suitable promoter, expression either stable or transient have utility. Finishing is done by downstream processing maximum protein extraction, costeffective processing with purification is yet a goal to be achieved.