Journal of Nanomedicine & Nanotechnology

Abstract

The Comparison of Silver and Hydroxyapatite Nanoparticles Biocompatibility on L929 Fibroblast Cells: An In vitro Study

Hossein Shahoon, Roya Hamedi, Zahra Yadegari, Vahid Al Majd Hosseiny, Pantea Golgounnia and Sogol Amiri

Background and aims: Nano particles are tiny materials (<1000 nm in size) that have specific physicochemical properties different to bulk materials of the same composition and such properties make them very attractive for commercial and medical development. In contrast to many efforts aimed at exploiting desirable properties of nano particles for medicine, there are limited attempts to evaluate potentially undesirable effects of these particles. Therefore, there is a pressing need for careful consideration of benefits and side effects of the use of nano particles in medicine. The purpose of this research was to compare the nano silver and hydroxyapatite nanoparticles’ biocompatibility on L929 fibroblast cells.
Materials and methods: To evaluate the biocompatibility of nano silver and hydroxyapatite nanoparticles (nHA), L929 fibroblast cells were cultured on a 96-well plate. Cells were exposed to nHA and nano silver at the following concentrations: 5, 10, 20, 30, 40, 50 ppm after 24, 48 and 72 hrs. Later, for measuring the biocompatibility of materials, MTT method was utilized.ANOVA test was used for statistical analysis.
Results: None of the nHA experimented concentrations were toxic, but vitality of cells exposing to nano silver particles were the least after 24 hours and increased after 48 hours and ANOVA analyze indicated that there was significant difference between groups (p<0.05). Also the result showed nano silver particles at concentrations more than 20 ppm within 24 and 48 hours are toxic to fibroblast cells and it was significant (p<0.02).
Conclusion: NHA were more biocompatible rather than nano silver particles on L929 fibroblast cells.