The fungicide PCZ (propiconazole) is widely used in agriculture especially in Asian contries as India, China ect. for the production of vegetable crops. Because of its physical and chemical properties , the small concentration of PCZ in water bodies “habitate of aquatic flora and fauna” make it sever in conditions for survivle. On the basis of analysis of all data in present study, the central theory that environmentally relevant conentrations of PCZ affect biochemical parameters in fish. The conclusion also hold that the theories such as metabolites of trizole convert the enzyme activities also. The LC values (LC50) estimate on different life stages of fish that was dose as well as time dependent. The exposure of sub-lethal concentration of PCZ in vivo assessment studied after 24 h and 72 h during exposure with 40% and 80% of LC50 (0.56 mg/l, 1.12 mg/l for fingerlings respectively) & (1.11 mg/l, 2.23 mg/l for adults respectively). Protein, Amino acids, Glycogen, Nucleic acids and enzyme succinic dehydrogenase decreased in liver and muscles, but lactic dehydrogenase levels, Protease, GOT and GPT increased in the both tissues. The study shows that PCZ have potential to damage aquatic ecosystem. Therefore, we can say that this fungicide should avoid in near water bodies.