Probiotics are good or friendly bacteria that are essential for good health. Probiotic literally means “for life” as opposed to antibiotic meaning “against life.” Probiotics are single-celled lactic bacteria organisms occurring primarily singly or in pairs. Probiotics are live microbial food supplements or components of bacteria, which have been shown to have beneficial effects on human health. The most commonly used probiotic strains belong to genera Lactobacillus sp., Bifidobacterium and Saccharomyces sp. taking into consideration, the above cited facts, and the present study was conducted to isolate and characterise probiotic strains from dairy waste water. Untreated and treated dairy waste water samples were collected from Jaipur dairy in accordance with standard procedures. Morphological and biochemical tests were conducted to identify the strains. The most common and predominant isolate was found to be associated with genera Leuconostoc sp.