Journal of Clinical & Experimental Pharmacology

Abstract

Protective Effect of Punica granatum L. on Gentamicin Induced Acute Renal Failure in Adult Rats

Rathod NS, Halagali KS, Nidavani RB, Shalavadi MH, Biradar BS, Biswas D, Chandrashekhar VM and Muchchandi IS

Aim: Study was undertaken to evaluate the effect of Punica granatum L. on Gentamicin-induced renal failure in rats. Materials and methods: Gr. I rats served as normal, received 0.5 ml of 5% Tween-80 in distilled water, Gr. II was injected with Gentamicin (100 mg/kg, IP), Gr. III was injected with Gentamicin and selenium (2 mg/kg, IP), and Gr. IV-IX were given orally Punica granatum fruit chloroform extract (PGCE) and Punica granatum methanol extract (PGME) at the doses of 100, 200 and 400 mg/kg, respectively, were administered for eight days in Gentamicininduced renal failure rats. On last day, Blood and 24 h urine was collected and used for estimation of serum and urine creatinine, urea, uric acid levels. The kidney homogenate was used for the estimation of LPO, SOD, CAT and GSH levels and Kidney sections were analyzed for histopathology. Results: Gentamicin-induced (Gr. II) had significant increase in levels of serum and urine creatinine, urea, uric acid, lipid peroxidation and significantly decrease in SOD, CAT and GSH levels as compared to normal (Gr. I). The treatment of PGCE and PGME 100, 200 and 400 mg/kg doses (Gr. IV-IX), significantly decreases serum and urine creatinine, urea, uric acid and significantly increases SOD, CAT and GSH levels in kidney homogenate with significant decrease in lipid peroxidation as compared to Gr. II. Conclusions: The PGCE and PGME at the doses of 400 mg/kg, found to be more effective in protecting the Gentamicin-induced renal failure in rats.