The study aimed to determine the prevalence, etiological agents and drug susceptibility pattern of bacterial pathogens isolated from postoperative surgical site infections and hospital environment in Gondar University Teaching Hospital. Specimens were taken from patients with post operative surgical site infections seen at wards and from hospital environment. Specimens were processed for bacteriological analysis and antibiotic susceptibility test according standard procedures. Antimicrobial susceptibility test for isolated organisms was done using disk diffusion method. Of 111 pathogenic bacteria, Escherichia coli 27 (24.3%), Staphylococcus aureus 26 (23.4%), Coagulase negative Staphylococci 22 (19.8%) and Enterobacter spp. 11 (9.9%) were dominant isolates. The prevalence of methicillin resistant S. aureus was 9 (34.6%). Seventeen (77.3%) and 1 (4.5%) of coagulase negative Staphylococcus were methicillin and vancomycin resistant, respectively. Coagulase negative Staphylococcus 41 (41.8%), S. aureus 19 (19.4%) and P. aeruginosa 16 (16.3%) were the major isolates from 75 sites of the hospital environment with an isolation rate of 41 (54.7%). The prevalence of methicillin resistant S.aureus from the environment was 2 (2.0%). High level of multi-drug resistance was observed in bacteria isolates from patients compared to the bacteria isolated from the hospital environments. This study demonstrated high level of multi-drug resistance. Thus, antibiotic sensitivity testing should be carried out for all bacterial isolates of surgical wounds before chemotherapy administered to detect drug resistant strains.