Biswajit Behuria, Sudip Ghimere, Suprabha Choudhary, Mohan Agrawal, Rabi Prasad Bodroth, & Thadepalli Venu Gopala Rao
The total phospholipids analyses had been performed in “Saccharomyces cerevisiae”, which is a baker’s yeast. This yeast is very much rich in carbohydrates, proteins, nucleic acids, and lipids, where these basic biomaterials (biological polymers) placed this haploid yeast as “single cell protein” category. Out of all phospholipids (PC, PE, PS, and PI) the world scientists have diverted their concentration towards PI and their metabolic components (e.g., PIP, PIP, PIP3 etc.,) and their applications on further growth of this baker’s yeast. Here, in this communication the PI levels were up to the mark on the Silica Gel-G (40 grams mixed in 60 ml of sterile distilled water) TLC plates. Further, the experimentation was extended in the growth levels observation of this haploid yeast in presence of isolated PIs (because of the existed assumption that the PIP, PIP2, & PIP3 are very much nearer in the surrounding of PI spot on Silica Gel-G plates), that may have enhanced the growth of S. cerevisiae. In addition, on supplementation of PI with S. cerevisiae grown cells stimulated the concentration of other Phospholipids includes PI. These results in total suggested that PI-supplemented cells have shown the stimulated growth and total phospholipids contents in this S. cerevisiae. It may be extrapolated that the second messenger PI and its metabolic intermediates may be involved as the remedy for phospholipids metabolic errors that lead to different deficient diseases. In final bit of experimentation the PI was isolated in UV exposed S cerevisiae cells which will further clarify metabolic changes and genetic deficiencies of PI metabolism.
Published Date: 2018-02-12;