Global Journal of Biology, Agriculture & Health Sciences

Abstract

PHENOTYPIC CHARACTERIZATION AND GENETIC VARIATION DETECTION USING ARBITRARY PRIMED POLYMERASE CHAIN REACTION FINGER PRINTING ON FOUR INDONESIAN WILD MONASCUS SPP Nandang

Nandang Suharna

The aim of this study was to know the phenotypic characters and also genetic variation by using arbitrary primed polymerase chain reaction finger printing of four wild Monascus spp. The phenotypic study showed Monascus sp. MYOT and Monascus sp. MYOM and Monascus sp. COEL had similar phenotypic characters. While Monascus sp. KTB was more different from the three Monascus isolates and considered different species. Although M. pilosus was regarded as the closest species but the four Monascus isolates were seemed to be new species. The AP-PCR analysis of the four Monascus isolates produced 124 DNA bands recognized visually. The phylogenetic tree generated showed the differences among the four isolates studied. This study indicated that use of APPCR is more suitable for isolates or strains differentiation rather than species differentiation. Further molecular study is needed to be carried out to determine its species name for the four Monascus isolates such as by using ITS gene sequence or other structural genes.

Published Date: 2013-11-04;