PMC/PubMed Indexed Articles
Indexed In
- Open J Gate
- Genamics JournalSeek
- JournalTOCs
- China National Knowledge Infrastructure (CNKI)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- MIAR
- Euro Pub
- Google Scholar
Useful Links
Share This Page
Journal Flyer
Open Access Journals
- Agri and Aquaculture
- Biochemistry
- Bioinformatics & Systems Biology
- Business & Management
- Chemistry
- Clinical Sciences
- Engineering
- Food & Nutrition
- General Science
- Genetics & Molecular Biology
- Immunology & Microbiology
- Medical Sciences
- Neuroscience & Psychology
- Nursing & Health Care
- Pharmaceutical Sciences
Abstract
Optimization of RAPD-PCR Protocol to Screen Jatropha Curcas and Gossypium Hirsutum Grown in Metal Contaminated Soil
Narayanan Mathiyazhagan and Devarajan Natarajan
The optimization of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) protocol was performed to screen two plants ( Jatropha curcas & Gossypium hirsutum ) grown in metal contaminated soils. A CTAB method was employed for DNA extraction, but an overnight RNase treatment was carried out to eliminate RNA contaminants. The isolated DNA was used for RAPD-PCR amplification. The optimum condition for reliable amplification requires a higher concentration of MgCl 2 (3 mM), primer (2.5 μM), Taq DNA polymerase (1 unit) and 3μl of template DNA (sample) and an annealing temperature of 55°C. Reproducible amplified products were observed in these conditions for both the plant species.