Journal of Nanomedicine & Nanotechnology

Abstract

Novel Trend in Colon Cancer Therapy Using Silver Nanoparticles Synthesized by Honey Bee

Nehal M El-Deeb, Ibrahim M El-Sherbiny, Mohamed R El-Aassara and Elsayed E Hafez

Recently, the biosynthesis of nanoparticles has been well explored which draws attention to its possible biomedical applications especially in cancer therapy. In the current study, the novelty in the biosynthesis of silver nanoparticles (AgNPs) using honey bee extract has been explained. This study was also aiming at investigating the anti-colon cancer activities of the biogenic AgNPs along with its capping biomolecules in vitro. The obtained biogenic AgNPs were well characterized by X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). It was found that the formed AgNPs have spherical shape with size range from 12 to 18 nm embedded in honey bee biomolecules. The cytotoxicity results of AgNPs on peripheral blood mononuclear cells (PBMC) indicated that the obtained AgNPs could be used safely with concentrations upto 39 μg/ml. On the other hand, the potentialities of the biogenic AgNPs against colon cancer proliferation recorded 60% inhibition using its nontoxic dose with a down regulation of the expression of Bcl2 and survivin gene. By the extraction of AgNPs capping biomolecules to explain the exact fraction that is responsible for the anticancer properties, it was found that both AgNPs and its capping biomolecules have anti-proliferative effects with a priority to the naked AgNPs.