Biochemistry & Analytical Biochemistry

Abstract

Nanoparticulat Printex 90 and Titanium Dioxide Stimulate Catecholamine Production in Ciliated Protozoan, Tetrahymena thermophila

Asad Ud-Daula and Karl-Werner Schramm

The aim of this study was to find the effect of printex 90 and titanium dioxide nanoparticle on catecholamine homeostasis in Tetrahymena thermophila. These two nanoparticles were exposed for 20 hours and 40 hours respectively. Tetrahymena cell produce catecholamine which was extracted by solid phase extraction and then analyzed by HPLCECD. Nanoparticles cause the stimulation of catecholamine production to the Tetrahymena. Noradrenaline and dopamine were predominantly synthesized in the cell with exposure of nanoparticles. Printex 90 induces strong stimulation of cell for catecholamine synthesis as compared to titanium dioxide. The production level of catecholamine increases with higher doses of nanoparticles with few exceptions. Nevertheless, this study clearly claims that nanoparticle increases catecholamine synthesis and causes subsequent toxicity.