Journal of Nanomedicine & Nanotechnology

Abstract

Impedance Spectroscopy of Supported Multiwalled Carbon Nanotubes for Immunosensor Applications

Imen Hafaiedh, Sami Ameur and Adnane Abdelghani

In this work, we used carboxyl multiwall carbons nanotubes for immunosensor application. The carbon nanotubes functionalisation can be achieved with Pressure Vapor Deposition (PVD) technique. The obtained carbon nanotubes can be immobilized on gold electrode with the airbrushing technique. The morphology of the deposited carboxyl carbon nanotubes was observed with Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The electrochemical characterization of the carboxyl multiwall carbon nanotubes was achieved with cyclic voltammetry and impedance spectroscopy. It shows a higher resistance and a good stability in water interface. For anti-rabbit IgG immobilization, the carboxyl carbon nanotubes were activated with EDC/NHS and non-specific sites was blocked with Bovine Serum Albumin (BSA). The development of immunosensor for IgG detection was observed with the impedance spectroscopy. The results show that the immunosensor response depends on the conductivity and the large surface-tovolume ratio attained with multiwall carbon nanotubes. The response of the developed immunosensor was reproducible, with higher stability and with limit detection of 10 pg/ml antigen.