This study is focus on Homology modelling of few Leguminosae family species which are found in Gujarat state, INDIA. There are three subfamilies of Leguminosae family which are Fabaceae (Papilionaceae), Caesalpiniaceae and Mimosaceae. Multiple sequence alignment carried out of few species’ rbcL protein sequences in each subfamily and conserved amino acid considered for homology modelling. Evolutionarily related proteins have similar sequences and naturally occurring homologous proteins have similar protein structure. It has been shown that three-dimensional protein structure is evolutionarily more conserved than would be expected on the basis of sequence conservation alone; we found that there are few amino acids which are common with same base pairs in each sub-family even though they are from different genus. There is no Protein structure available of conserved amino acids in PDB database of our study so we did homology modelling of three rbcL protein sequences (one from each sub family) which are found conserved in Multiple sequence alignment and structure validation with Ramachandran Plot was carried out and CASTp server was used to find out active sites in predicted protein structure and finally function of each predicted protein reported after this homology modeling of few conserved rbcL amino acid sequences in Leguminosae family.