Journal of Petroleum & Environmental Biotechnology

Abstract

Exploitation of Two Consortiums in Microbial Degradation and Decolorization of Remazol Black and Acid Orange

Shah M

Different soil samples were collected from effluent treatment plants were used as a source for the isolation of 16 morphologically distinct bacteria capable of decolourising textile dye, Acid orange. Decolourisation assay was performed using two textile dyes - Acid oragne and emazol black to screen for high dye decolourising bacterial isolates. 5 Bacterial isolates showed high decolourisation of Acid orange and a consortium of these 5 colonies called A was developed. 3 Bacterial isolates showed high decolourisation of Remazol black and a consortium of these 3 isolates, B, was developed. The screened bacterial isolates were identified as Pseudomonas sp., Alcaligens sp., Rhodococcus sp., Stenotrophomonas sp., Proteobacterium sp., and Bacillus sp. by 16S rDNA analysis. A exhibited high decolourisation percentage of 200 mg/l of Acid orange in the presence of anhydrous sodium acetate under static condition at pH 7 and temperature of 40°C within 48 hours. B showed high decolourisation of 200 mg/l of Remazol black in the presence of galactose and a combination of straw and beef extract under static condition at pH 7 and temperature of 30°C within 48 hours. LC-MS analysis of the degraded product of Acid orange by A showed the presence of 1-{3-amino 5- [(aminoxy) sulphonyl] phenyl} ethanol and 7, 8-amino-3-[(aminoxy) sulphonyl] napthalen- 1-ol. LC-MS analysis of the degraded product of Remazol black by B showed the presence of 1.1’-diazene-1,2- diyldinapthlen-2-ol and sodium-4-amino-naphthalene-1-sulphonate.