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Age-dependent chronic and aggressive periodontitis patients need renewal of soft and hard tissue like periodontal ligament, gingiva, cementum and alveolar bone. Hence, multipotent-Mesenchymal Stromal Cells (MSCs) of different dental origin like Dental Pulp (DP); Periodontal Ligament (PDL); Gingiva; Apical Papilla (AP) may play important role in regeneration of periodontal tissue. For the accomplishment of this objective, we standardized and validated the manufacturing process of dental-MSCs sources like DP, PDL, AP and gingiva from the subjects ranging from the age of 13 to 60 years. As per, International guidelines, we isolated and enumerated plastic-adherent MSCs at different time-points from different dental sources, phenotypically characterized these cells and differentiated them into osteogenic, adipogenic, and chondrogenic cells. Importantly, in subjects from 13-31 years copious DP gave an appropriate amount of MSCs. While, between the age group 50-60 years negligible amount of DP was extracted, which did not provide any MSCs. However, PDL and gingiva produced a good amount of MSCs from 13-31 years, but in 50-60 years the yield was not good. Hence, age does affect MSCs number which is the reason of delayed healing in older age group subjects compared to younger subjects.