Awards Nomination 20+ Million Readerbase
Indexed In
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • JournalTOCs
  • CiteFactor
  • Ulrich's Periodicals Directory
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • Centre for Agriculture and Biosciences International (CABI)
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • Google Scholar
Share This Page
Journal Flyer
Flyer image

Abstract

Development of Simple and Rapid Diagnostic Method for Strawberry Latent Ring-spot Virus in Plants Using Loop-Mediated Isothermal Amplification Assay

Jin-Ho Kim, Siwon Lee, Ji-Young Choi, Sue Kyung Kim and Won-Cheoul Jang

Strawberry Latent Ring-spot Virus (SLRSV) is seed or nematodes-transmitted viruses, and causes quantitative and qualitative loss of various crops. SLRSV is a non-reported, potentially control able virus, which is managed at the national level. Currently, RT-PCR and nested PCR system are the standard methods of detecting SLRSV, but more effective methods are required. In this study, loop-mediated isothermal amplification (LAMP) assay was used for detection of SLRSV. As a result, the LAMP assay showed sensitivity similar to that of the currently used method, but is more rapid (approximately 8 hrs), simple and specific. In addition, results can be verified by restriction fragment length polymorphism (RFLP) using BfaI, or sequencing after the LAMP reaction. Therefore, we have shown that the LAMP assay developed in this study is a potential marker for the facilitation of rapid and simple screening of SLRSV in plants, which will ultimately be useful for the diagnosis of SLRSV infected plants and quarantine.