Pharmaceutica Analytica Acta

Abstract

Development of a Rapid HPLC-UV Method for Analysis of Menaquinone-7 in Soy Nutraceutical

Rishipal S, Alka P, Mojeer H and Bibhu Prasad P

Among all fermented foods, fermented soybeans (soy nutraceutical) were found wide applications as nutraceutical due to nutritional benefit. The vitamin K2, menaquinone-7 (MK-7) is commonly used for preventing osteoporosis and is an important secondary metabolite of soy nutraceutical. A consistent and improved highperformance liquid chromatography (HPLC) method for determination of MK-7 in fermented soybean is developed. The analysis was achieved on Lichrospher-100, RP-C18 (5 μm) column with a dimension 125 mm × 4.0 mm, with detection at 248 nm using a gradient mobile phase mixture of water and methanol (1:1 v/v) acidified to pH 3.0 by orthophosphoric acid and acetonitrile with a flow rate of 1.2 mL min-1. Under these conditions, the analysis of MK-7 was achieved in less than 4 min. The retention time was found to be 2.38 min. The calibration curve for MK-7 was linear in the range of 2.5-20 μg mL-1 with R2=0.9997. The proposed method was successfully employed for quantification of the MK-7 present in soy nutraceutical.