Background and aim: Although antibiotics are used in the treatment of infectious diseases nevertheless, there are many problems such as adverse drug reactions and resistance to antibiotics. Plants, which may have less adverse reactions, can be suitable substitute for chemical drugs. Cichorium intybus L.which is one of the herbs that can be easily found in many areas of Iran, has antibacterial effect and can be used in the treatment of infectious diseases. The present study was planned for comparison of antibacterial effect of Cichorium intybus L. with vancomycin, ceftriaxone, ciprofloxacin and penicillin. Methods: In the present experimental study Streptococcus pyogen, Staphylococcus aureus and Enterococcus were cultured on blood-agar medium. Alcoholic extract of Cichorium intybus L. (AECI) was added to culture media along with antibiotics (vancomycin, ceftriaxone, ciprofloxacin and penicillin) discs. Zone of inhibition of samples were measured and the data was analyzed by using Chi square and Fisher΄s exact tests. Results: AECI had no antibacterial effect on the respective microorganisms, whereas Vancomycin had antibacterial effect on Staphylococcus aureus and Streptococcus pyogen but no effect on Enterococcus. Ceftriaxone had antibacterial effect on Enterococcus and Streptococcus pyogen with no effect on Staphylococcus aureus. Ciprofloxacin had antibacterial effect on Enterococcus and Staphylococcus aureus and Penicillin had only effect on Streptococcus pyogen. Conclusion: AECI had no antibacterial effect on Streptococcus pyogen, Staphylococcus aureus and Enterococcus. We conclude that other extracts of Cichorium intybus L. such as aqueous or ethyl acetate may have antibacterial effect on gram positive bacteria which requiring more studies to prove.