Journal of Bacteriology & Parasitology

Abstract

Comparison of Acinetobacter baumannii multidrugs resistant Isolates obtained from French and Tunisian hospitals

Ben Othman A, Burucoa C, Battikh H, Zribi M, Masmoudi A and Fendri C

Objectives: The aim of this study was to assess whether there are differences between the clustering of isolates collected between 2003 and 2005 from two different hospitals in Tunisia and France. Methods: A selection of 62 isolates of A. baumannii was studied; 31 from the French Hospital (Poitiers) and 31 from Tunisian Hospital (Rabta). Antibiograms were done using the disc diffusion method. The presence of integrons class 1 and 2 was studied by PCR. Molecular relationship was studied by Random Amplified Polymorphic DNA (RAPD) and Pulsed Field Gel Electrophoresis (PFGE). The sequence typing of adeB gene was determined to identify intraspecific groups. Results: The present study successfully focuses to compare epidemiologic status between two hospitals study. PFGE and RAPD methods were useful to distinguish epidemic and endemic clones in the two collections study. Sequence analysis of an 850-bp internal fragment drug efflux gene adeB revealed 9 novel sequence types (STs). Conclusion: We, statistically, found no significant difference related to the epidemiological situation. This study showed that different genetic types of A.baumannii were found in the two collection strains. Epidemics ones were essentially confined in the ICU and were persisting during the two years study. However, more control procedures had to be used on these clones for the Tunisian hospital.