Anup Baranwal, Jeevana Praharsha, Ramanuj Rauniyar, Rabindra Khadka, Ramprasad Kuncham & Vinod Nagashetti
The Globe has been encountering a large number of problems related to the various ill management of various resources due to extensive industrialization, increasing population density and a highly urbanized society because of this there is a large amount of effluents is responsible for the degradation of water bodies, Soils and the environment In this study the Novel microorganism are used for the removal of the toxic and heavy metals, despite of being their own relevant properties. Various microorganisms is known to accumulate metal in their cell or cell walls either in the viable or attenuated state by the process called Biosorption .In this study we have targeted the three metals ions such as the Lead, Cobalt and Chromium .The Microorganism used are the Bacteria E.coli-ATCC12458 and the Pseudomonas aeruginosaATCC-15457 and the Fungui are the Saccromyceae .cerevisiae NCL-3570, Pichia stipitis NCL-3497and the Tricoderma resi NICM-4596. Among the techniques appropriate for the quantification of metal ions in broth sample, inductively coupled plasma mass spectrometry are likely to be the most widely employed. However, although these techniques are unfailing and sensitive, they endure from the limitation of being rather costly (considering instrument acquisition and maintenance), time-consuming (with respect to sample preparation), and not always readily available. Therefore a general titration based analysis is been performed for the lead, cobalt and spectroscopic analysis for the chromium. The results are quite promising, Metal absorbed by E.coli ,Pseudomonas aeruginosa , ,Tricoderma.resi, S. cerevisiae and Pichia stipitis i.e., Cr, Co and Pb in 1000 ppm was 735ppm, 715ppm,736ppm,745ppm &630ppm for the Chromium ,for the lead it is 750ppm,748ppm,785ppm,755ppm &715ppm and for the cobalt it is 698ppm,715ppm,765ppm,715ppm &690ppm and respectively for the others when compared to control broth sample in 500ppm &250ppm . The present study depicts that bacteria and fungus removes chromium, cobalt and lead efficiently and this could be used for industrial waste management and other environmental contaminants. Therefore it is been concluded that apart from their Novel activities these microorganism can be used as a toxic and metal removing agent.