Modern Chemistry & Applications

Abstract

Assessing the Quality of Various Preparations of Calendula officinalis using High Performance Thin Layer Chromatography

Snezana Agatonovic-Kustrin, Anindita Chakrabarti, David W Morton and Pauzi A Yusof

The primary goal of this study was to develop a simple and reliable High-Performance-Thin-Layer Chromatography method to quantitate active ingredients in commercial topical formulations containing Calendula officinalis extract and to investigate the affect of different extraction solvents on the overall quality of the formulation. The developed method was validated for linearity, precision, accuracy, limit of detection, and limit of quantification. It was found that commercially available formulations containing extracts of Calendula officinalis have significant variations in both composition and amounts of active pharmaceutical ingredients, due to different extraction procedures employed and the standardization requirements for extracts used in formulation.